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New Zealand Retinoblastoma
Gene Testing Service - Samples
DNA analysis requires:
- Consent Forms for gene testing and possible research.
- 10mls EDTA blood (purple top tube ) from patient.
- 10mls EDTA blood from both parents.
- Consent forms to be signed by legal guardians.
- 5 mls blood in Lithium-Heparin tube (green top) to be sent to appropriate
cytogenetic laboratory for chromosome or FISH analysis.
- Legal guardians to receive appropriate genetic counselling on implications
of DNA testing.
- Tumour sample to be frozen immediately in liquid nitrogen. The tumour
must be carefully dissected without blood or normal tissue contamination.
Care must also be given to avoiding tumour material contaminating the
optic nerve before histological analysis.
Once removed the tumour is placed in a cryo-vial, which is then immediately placed in liquid
nitrogen. After snap freezing the sample can then be stored at -80oC or in dry ice. The vial can then
be shipped in dry ice on an overnight delivery to:
Dr Anthony Raizis
Department of Molecular Pathology,
c/o Canterbury Health Laboratories,
43 St Asaph St,
Christchurch
Download Consent Forms:
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