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Free Radical Research
Group - Assays
Protein Carbonyl ELISA
An ELISA based on the reaction of protein carbonyls with
dinitrophenylhydrazine (DNP) and detection with an antibody against DNP.
This assay is available in kit form, marketed by our partners, BioCell
Corporation. Details on the kit and information about purchasing are
available on the BioCell web site.
Kit Developed By:
I. Hendrikje Buss, Christine C. Winterbourn and co-workers at the Free Radical
Research Group, Christchurch School of Medicine and Health Sciences (University of Otago), Christchurch, New Zealand.
Distributed By:
BioCellCorporation Ltd.,
Papatoetoe, New Zealand
Phone +64 9 270 0755
Fax +64 9 270 0751
Website: BioCell Corporation
References
- Protein carbonyl measurement by a sensitive ELISA method. H. Buss,
T.P. Chan, K.B. Sluis, N.M. Domigan, C.C. Winterbourn; Free Radic Bio Med
1997;23(3):361-6.
- Protein carbonyl measurement by a sensitive
ELISA method, Erratum .Buss, H., Chan, T.P., Sluis, K.B., Domigan,
N.M., and Winterbourn, C.C., Free Radic Bio Med 24(7/8): 1352, 1998.
- Protein carbonyl measurement by enzyme-linked immunosorbent assay.
C.C.Winterbourn, I.H. Buss; Methods Enzymol 1999;300:106-11.
- Small Bowel ischaemia-reperfusion increases plasma concentrations of
oxidized proteins in rats. F.M. Abu-Zidan, C.C. Winterbourn, M.J.D. Bonham,
M.O. Simovic, H. Buss, J.A. Windsor; Eur J Surg 1999 Apr;165(4):383-9.
- Protein carbonyl measurements show evidence of early oxidative stress in
critically ill patients. C.C. Winterbourn, I.H. Buss, T.P. Chan, L.D. Plank,
M.A. Clark, J.A. Windsor; Crit Care Med 2000 Jan;28(1):143-9.
- Elevated protein carbonyls, lipid peroxidation products and myeloperoxidase
in tracheal aspirates from premature infants; I.H. Buss, B.A. Darlow, C.C.
Winterbourn; Pediatr Res 2000 May 47(5) p 640-5.
- Protein carbonyls and lipid peroxidation products as oxidation markers in
preterm infant plasma: Associations with chronic lung disease and retinopathy
and effect of selenium supplementation; C.C. Winterbourn, T.P. Chan, I.H. Buss,
T.E. Inder, N. Mogridge, B.A. Darlow; Pediatr Res 2000;48(1):84-90
Neutrophil Killing Assay
Rates of bacterial phagocytosis and killing by neutrophils
can be calculated using straightforward mathematical analyses of data
obtained according to Journal of Immunological Methods
232 (1999) 15-22 and Green et al. in
chapter 21, Methods in Molecular Biology – Neutrophil
methods and protocols (2006).
An Excel file to carry out these calculations and instructions for
use can be downloaded (45 Kb
zip file). Any queries,
please contact Mark
Hampton.
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